Gill:TSA (Tryptone Soy Agar): Difference between revisions
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(New page: ==Materials== 500 mL: *TSB (Bacto) 15 g *dH<sub>2</sub>O 500 ml 1000 mL: *TSB (Bacto) 30 g *dH<sub>2</sub>O 1000 ml ==Procedure== Dissolve medium in water. Add 7.5 g Bacto agar...) |
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| Line 3: | Line 3: | ||
500 mL: | 500 mL: | ||
*TSB (Bacto) | *15 g TSB (Bacto) | ||
*dH<sub>2</sub>O | *500 ml dH<sub>2</sub>O | ||
1000 mL: | 1000 mL: | ||
*TSB (Bacto) | *30 g TSB (Bacto) | ||
*dH<sub>2</sub>O | *1000 ml dH<sub>2</sub>O | ||
==Procedure== | ==Procedure== | ||
Revision as of 14:05, 23 April 2013
Materials
500 mL:
- 15 g TSB (Bacto)
- 500 ml dH2O
1000 mL:
- 30 g TSB (Bacto)
- 1000 ml dH2O
Procedure
Dissolve medium in water. Add 7.5 g Bacto agar to empty 1 L bottles, aliquot 500 ml of medium to each bottle. Label caps with “TSA” to avoid confusion. Immediately after autoclaving, swirl media to dissolve the melted agar. Tighten caps and place bottles in the water bath. Pour plates when the agar temperature is 50-60 ºC.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
