Gill:TNB (Tryptone Nutrient Broth)
- Tryptone 2.5 g
- Yeast extract 1.25 g
- Glucose 0.5 g
- NaCl 4.25 g
- dH2O 500 ml
Mix all ingredients in a 1 litre bottle or flask, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!