Gill:TNB (Tryptone Nutrient Broth): Difference between revisions
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(New page: ==Materials== 500 mL: *Tryptone 2.5 g *Yeast extract 1.25 g *Glucose 0.5 g *NaCl 4.25 g *dH<sub>2</sub>O 500 ml ==Procedure== Mix all ingredients in a 1 litre bottle or flask,...) |
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| Line 3: | Line 3: | ||
500 mL: | 500 mL: | ||
* | *2.5 g Tryptone | ||
* | *1.25 g Yeast extract | ||
* | *0.5 g Glucose | ||
* | *4.25 g NaCl | ||
*dH<sub>2</sub>O | *500 ml dH<sub>2</sub>O | ||
==Procedure== | ==Procedure== | ||
Latest revision as of 13:56, 23 April 2013
Materials
500 mL:
- 2.5 g Tryptone
- 1.25 g Yeast extract
- 0.5 g Glucose
- 4.25 g NaCl
- 500 ml dH2O
Procedure
Mix all ingredients in a 1 litre bottle or flask, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
