Gill:Supplemented M9 broth (M9sup)

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Materials

50 mL 500 mL 1000 mL
dH2O 43 ml 430 ml 860 ml
10 X M9 salts + glucose 5 ml 50 ml 100 ml
10% Casamino acids 1 ml 10 ml 20 ml
1% Thiamine 50 µl 0.5 ml 1 ml
1 M MgSO4 100 µl 1 ml 2 ml
1 M CaCl2 5 µl 50 µl 0.1 ml

Procedure

Pre-sterilize all component solutions and aseptically add to sterile water. Alternatively, mix all components and then filter-sterilize through a 0.22 µm filter. Store at 4 ºC.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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