Gill:S liquid medium

From OpenWetWare

Revision as of 16:08, 23 April 2013 by Jacqueline K. Grimm (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Contents

Overview

For Bcc EPS production.

Materials

1000 mL:

  • 1 g Yeast extract
  • 10 g Na2HPO4
  • 3 g KH2PO4
  • 1 g K2SO4
  • 1 g NaCl
  • 900 mL Water

Procedure

Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:

20 % Glucose 100 ml (2% final)
10 % Casamino acids 10 ml (0.1% final)
MgSO4 (from 1 M stock) 1 ml (1 mM final)
CaCl2 (from 1 M stock) 0.1 ml (0.1 mM final)
FeSO4 (from 0.1 M stock) 50 µl (5 µM final)

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools