Gill:S liquid medium
For Bcc EPS production.
- Yeast extract 1 g
- Na2HPO4 10 g
- KH2PO4 3 g
- K2SO4 1 g
- NaCl 1 g
- Water 900 mL
Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:
|20 % Glucose||100 ml||(2% final)|
|10 % Casamino acids||10 ml||(0.1% final)|
|MgSO4 (from 1 M stock)||1 ml||(1 mM final)|
|CaCl2 (from 1 M stock)||0.1 ml||(0.1 mM final)|
|FeSO4 (from 0.1 M stock)||50 µl||(5 µM final)|
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!