Gill:S liquid medium
For Bcc EPS production.
- Yeast extract 1 g
- Na2HPO4 10 g
- KH2PO4 3 g
- K2SO4 1 g
- NaCl 1 g
- Water 900 mL
Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:
20 % Glucose 100 ml (2% final)
10 % Casamino acids 10 ml (0.1% final)
MgSO4 (from 1 M stock) 1 ml (1 mM final)
CaCl2 (from 1 M stock) 0.1 ml (0.1 mM final)
FeSO4 (from 0.1 M stock) 50 µl (5 µM final)
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!