Gill:S liquid medium

Overview

For Bcc EPS production.

Materials

1000 mL:

• Yeast extract 1 g
• Na₂HPO₄ 10 g
• KH₂PO₄ 3 g
• K₂SO₄ 1 g
• NaCl 1 g
• Water 900 mL

Procedure

Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:

20 % Glucose 100 ml (2% final)
10 % Casamino acids 10 ml (0.1% final)
MgSO₄ (from 1 M stock) 1 ml (1 mM final)
CaCl₂ (from 1 M stock) 0.1 ml (0.1 mM final)
FeSO₄ (from 0.1 M stock) 50 µl (5 µM final)

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!