Gill:S liquid medium

Overview

For Bcc EPS production.

Materials

1000 mL:

• Yeast extract 1 g
• Na₂HPO₄ 10 g
• KH₂PO₄ 3 g
• K₂SO₄ 1 g
• NaCl 1 g
• Water 900 mL

Procedure

Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:

20 % Glucose	100 ml	(2% final)
10 % Casamino acids	10 ml	(0.1% final)
MgSO4 (from 1 M stock)	1 ml	(1 mM final)
CaCl2 (from 1 M stock)	0.1 ml	(0.1 mM final)
FeSO4 (from 0.1 M stock)	50 µl	(5 µM final)

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!