Gill:S liquid medium

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
Current revision (17:08, 23 April 2013) (view source)
m
 
Line 6: Line 6:
1000 mL:
1000 mL:
-
*Yeast extract 1 g
+
*1 g Yeast extract
-
*Na<sub>2</sub>HPO<sub>4</sub> 10 g
+
*10 g Na<sub>2</sub>HPO<sub>4</sub>
-
*KH<sub>2</sub>PO<sub>4</sub> 3 g
+
*3 g KH<sub>2</sub>PO<sub>4</sub>
-
*K<sub>2</sub>SO<sub>4</sub> 1 g
+
*1 g K<sub>2</sub>SO<sub>4</sub>
-
*NaCl 1 g
+
*1 g NaCl
-
*Water 900 mL
+
*900 mL Water
==Procedure==
==Procedure==

Current revision

Contents

Overview

For Bcc EPS production.

Materials

1000 mL:

  • 1 g Yeast extract
  • 10 g Na2HPO4
  • 3 g KH2PO4
  • 1 g K2SO4
  • 1 g NaCl
  • 900 mL Water

Procedure

Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:

20 % Glucose 100 ml (2% final)
10 % Casamino acids 10 ml (0.1% final)
MgSO4 (from 1 M stock) 1 ml (1 mM final)
CaCl2 (from 1 M stock) 0.1 ml (0.1 mM final)
FeSO4 (from 0.1 M stock) 50 µl (5 µM final)

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools