Gill:S liquid medium: Difference between revisions
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(New page: ==Overview== For Bcc EPS production. ==Materials== 1000 mL: *Yeast extract 1 g *Na<sub>2</sub>HPO<sub>4</sub> 10 g *KH<sub>2</sub>PO<sub>4</sub> 3 g *K<sub>2</sub>SO<sub>4</sub> 1 g *Na...) |
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| Line 6: | Line 6: | ||
1000 mL: | 1000 mL: | ||
*Yeast extract | *1 g Yeast extract | ||
*Na<sub>2</sub>HPO<sub>4</sub> | *10 g Na<sub>2</sub>HPO<sub>4</sub> | ||
*KH<sub>2</sub>PO<sub>4</sub> | *3 g KH<sub>2</sub>PO<sub>4</sub> | ||
*K<sub>2</sub>SO<sub>4</sub> | *1 g K<sub>2</sub>SO<sub>4</sub> | ||
* | *1 g NaCl | ||
* | *900 mL Water | ||
==Procedure== | ==Procedure== | ||
Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions: | Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions: | ||
20 % Glucose 100 ml (2% final) | {| class="wikitable" | ||
10 % Casamino acids 10 ml (0.1% final) | |- | ||
MgSO<sub>4</sub> (from 1 M stock) 1 ml (1 mM final) | |20 % Glucose | ||
CaCl<sub>2</sub> (from 1 M stock) 0.1 ml (0.1 mM final) | |100 ml | ||
FeSO<sub>4</sub> (from 0.1 M stock) 50 µl (5 µM final) | |(2% final) | ||
|- | |||
|10 % Casamino acids | |||
|10 ml | |||
|(0.1% final) | |||
|- | |||
|MgSO<sub>4</sub> (from 1 M stock) | |||
|1 ml | |||
|(1 mM final) | |||
|- | |||
|CaCl<sub>2</sub> (from 1 M stock) | |||
|0.1 ml | |||
|(0.1 mM final) | |||
|- | |||
|FeSO<sub>4</sub> (from 0.1 M stock) | |||
|50 µl | |||
|(5 µM final) | |||
|} | |||
==Notes== | ==Notes== | ||
Latest revision as of 14:08, 23 April 2013
Overview
For Bcc EPS production.
Materials
1000 mL:
- 1 g Yeast extract
- 10 g Na2HPO4
- 3 g KH2PO4
- 1 g K2SO4
- 1 g NaCl
- 900 mL Water
Procedure
Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:
| 20 % Glucose | 100 ml | (2% final) |
| 10 % Casamino acids | 10 ml | (0.1% final) |
| MgSO4 (from 1 M stock) | 1 ml | (1 mM final) |
| CaCl2 (from 1 M stock) | 0.1 ml | (0.1 mM final) |
| FeSO4 (from 0.1 M stock) | 50 µl | (5 µM final) |
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
