Gill:S liquid medium

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: ==Overview== For Bcc EPS production. ==Materials== 1000 mL: *Yeast extract 1 g *Na<sub>2</sub>HPO<sub>4</sub> 10 g *KH<sub>2</sub>PO<sub>4</sub> 3 g *K<sub>2</sub>SO<sub>4</sub> 1 g *Na...)
m
Line 16: Line 16:
Dissolve above components in 900 ml water and autoclave.  Before use, add the following components from sterile stock solutions:
Dissolve above components in 900 ml water and autoclave.  Before use, add the following components from sterile stock solutions:
-
20 % Glucose 100 ml (2% final) <br>
+
{| class="wikitable"
-
10 % Casamino acids 10 ml (0.1% final) <br>
+
|-
-
MgSO<sub>4</sub> (from 1 M stock) 1 ml (1 mM final) <br>
+
|20 % Glucose  
-
CaCl<sub>2</sub> (from 1 M stock) 0.1 ml (0.1 mM final) <br>
+
|100 ml  
-
FeSO<sub>4</sub> (from 0.1 M stock) 50 µl (5 µM final)
+
|(2% final)
 +
|-
 +
|10 % Casamino acids  
 +
|10 ml  
 +
|(0.1% final)
 +
|-
 +
|MgSO<sub>4</sub> (from 1 M stock)  
 +
|1 ml  
 +
|(1 mM final)
 +
|-
 +
|CaCl<sub>2</sub> (from 1 M stock)  
 +
|0.1 ml
 +
|(0.1 mM final)
 +
|-
 +
|FeSO<sub>4</sub> (from 0.1 M stock)
 +
|50 µl  
 +
|(5 µM final)
 +
|}
==Notes==
==Notes==

Revision as of 16:47, 23 April 2013

Contents

Overview

For Bcc EPS production.

Materials

1000 mL:

  • Yeast extract 1 g
  • Na2HPO4 10 g
  • KH2PO4 3 g
  • K2SO4 1 g
  • NaCl 1 g
  • Water 900 mL

Procedure

Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:

20 % Glucose 100 ml (2% final)
10 % Casamino acids 10 ml (0.1% final)
MgSO4 (from 1 M stock) 1 ml (1 mM final)
CaCl2 (from 1 M stock) 0.1 ml (0.1 mM final)
FeSO4 (from 0.1 M stock) 50 µl (5 µM final)

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools