Gill:Protocols: Difference between revisions
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[[Gill:Confirming phage via gel electrophoresis |Confirming phage via restriction digest and gel electrophoresis]] | [[Gill:Confirming phage via gel electrophoresis |Confirming phage via restriction digest and gel electrophoresis]] | ||
[[Gill:Generation of double-amber phage mutants |Generation of double-amber phage mutants]] | |||
[[Gill:Making frozen permanents |Making frozen permanents]] | [[Gill:Making frozen permanents |Making frozen permanents]] | ||
Revision as of 11:52, 26 March 2015
Protocols
Microbiology and Molecular Biology
Calcium chloride competent cells
Confirming phage via restriction digest and gel electrophoresis
Generation of double-amber phage mutants
Preparing phage specimens for TEM
S. aureus genomic DNA isolation
S. aureus pre-treatment for plasmid preps
Solutions and Media Preparation
10x Lambda diluent (no gelatin)
