Gill:Protocols: Difference between revisions
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===Microbiology and Molecular Biology=== | ===Microbiology and Molecular Biology=== | ||
[[Gill:Chung cells |Chung cells]] | |||
[[Gill:Confirming phage via gel electrophoresis |Confirming phage via gel electrophoresis]] | [[Gill:Calcium chloride competent cells |Calcium chloride competent cells]] | ||
[[Gill:Making frozen permanents |Making frozen permanents]] | |||
[[Gill:S. aureus genomic DNA isolation |S. aureus genomic DNA isolation]] | [[Gill:Chung cells |Chung cells]] | ||
[[Gill:Confirming phage via gel electrophoresis |Confirming phage via restriction digest and gel electrophoresis]] | |||
[[Gill:Generation of double-amber phage mutants |Generation of double-amber phage mutants]] | |||
[[Gill:Making frozen permanents |Making frozen permanents]] | |||
[[Gill:Making plate lysates |Making plate lysates]] | |||
[[Gill:Phage enrichment |Phage enrichment]] | |||
[[Gill:Phage genomic DNA extraction |Phage genomic DNA extraction]] | |||
[[Gill:Plating out phage |Plating out phage]] | |||
[[Gill:Preparing phage specimens for TEM |Preparing phage specimens for TEM]] | |||
[[Gill:Rapid bacterial DNA prep |Rapid bacterial DNA prep]] | |||
[[Gill:S. aureus genomic DNA isolation |S. aureus genomic DNA isolation]] | |||
[[Gill:S. aureus pre-treatment for plasmid preps |S. aureus pre-treatment for plasmid preps]] | |||
[[Gill:Spot complementation test |Spot complementation test]] | |||
[[Gill:Subculturing phage isolates |Subculturing phage isolates]] | |||
===Solutions and Media Preparation=== | ===Solutions and Media Preparation=== | ||
Revision as of 11:52, 26 March 2015
Protocols
Microbiology and Molecular Biology
Calcium chloride competent cells
Confirming phage via restriction digest and gel electrophoresis
Generation of double-amber phage mutants
Preparing phage specimens for TEM
S. aureus genomic DNA isolation
S. aureus pre-treatment for plasmid preps
Solutions and Media Preparation
10x Lambda diluent (no gelatin)
