Gill:Protocols: Difference between revisions
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==Protocols== | ==Protocols== | ||
===Microbiology=== | ===Microbiology and Molecular Biology=== | ||
[[Gill: | [[Gill:Calcium chloride competent cells |Calcium chloride competent cells]] | ||
[[Gill:Chung cells |Chung cells]] | |||
[[Gill:Confirming phage via gel electrophoresis |Confirming phage via restriction digest and gel electrophoresis]] | |||
[[Gill:Generation of double-amber phage mutants |Generation of double-amber phage mutants]] | |||
[[Gill:Making frozen permanents |Making frozen permanents]] | |||
[[Gill:Making plate lysates |Making plate lysates]] | |||
[[Gill:Phage enrichment |Phage enrichment]] | |||
[[Gill:Phage genomic DNA extraction |Phage genomic DNA extraction]] | |||
[[Gill:Plating out phage |Plating out phage]] | |||
[[Gill:Preparing phage specimens for TEM |Preparing phage specimens for TEM]] | |||
[[Gill:Rapid bacterial DNA prep |Rapid bacterial DNA prep]] | |||
[[Gill:S. aureus genomic DNA isolation |S. aureus genomic DNA isolation]] | |||
[[Gill: | [[Gill:S. aureus pre-treatment for plasmid preps |S. aureus pre-treatment for plasmid preps]] | ||
[[Gill: | [[Gill:Spot complementation test |Spot complementation test]] | ||
[[Gill: | [[Gill:Subculturing phage isolates |Subculturing phage isolates]] | ||
===Solutions and Media Preparation=== | |||
[[Gill:Lambda diluent (SM buffer) |Lambda diluent (SM buffer)]] | [[Gill:Lambda diluent (SM buffer) |Lambda diluent (SM buffer)]] | ||
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[[Gill:10x Lambda diluent (no gelatin) |10x Lambda diluent (no gelatin)]] | [[Gill:10x Lambda diluent (no gelatin) |10x Lambda diluent (no gelatin)]] | ||
[[Gill: | [[Gill:LB agar |LB agar]] | ||
[[Gill:LB broth |LB broth]] | [[Gill:LB broth |LB broth]] | ||
[[Gill: | [[Gill:M9 agar |M9 agar]] | ||
[[Gill: | [[Gill:Supplemented M9 agar (M9sup) |Supplemented M9 agar (M9sup)]] | ||
[[Gill: | [[Gill:Supplemented M9 broth (M9sup) |Supplemented M9 broth (M9sup)]] | ||
[[Gill:10 X M9 salts |10 X M9 salts]] | [[Gill:10 X M9 salts |10 X M9 salts]] | ||
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[[Gill:10 X M9 salts + glucose |10 X M9 salts + glucose]] | [[Gill:10 X M9 salts + glucose |10 X M9 salts + glucose]] | ||
[[Gill: | [[Gill:S liquid medium |S liquid medium]] | ||
[[Gill:TNA (Tryptone Nutrient Agar) |TNA (Tryptone Nutrient Agar)]] | |||
[[Gill:TNA-SA |TNA-SA]] | |||
[[Gill:TNB (Tryptone Nutrient Broth) |TNB (Tryptone Nutrient Broth)]] | |||
[[Gill:Tris-HCl |Tris-HCl]] | |||
[[Gill:TSA (Tryptone Soy Agar) |TSA (Tryptone Soy Agar)]] | |||
[[Gill: | [[Gill:TSB (Tryptone Soy Broth) |TSB (Tryptone Soy Broth)]] | ||
[[Gill: | [[Gill:T-top (0.5% agar) |T-top (0.5% agar)]] | ||
[[Gill:Vogel-Bonner glucose (VBG) minimal medium |Vogel-Bonner glucose (VBG) minimal medium]] | [[Gill:Vogel-Bonner glucose (VBG) minimal medium |Vogel-Bonner glucose (VBG) minimal medium]] | ||
[[Gill:50X Vogel-Bonner salts |50X Vogel-Bonner salts]] | [[Gill:50X Vogel-Bonner salts |50X Vogel-Bonner salts]] |
Revision as of 11:52, 26 March 2015
Protocols
Microbiology and Molecular Biology
Calcium chloride competent cells
Confirming phage via restriction digest and gel electrophoresis
Generation of double-amber phage mutants
Preparing phage specimens for TEM
S. aureus genomic DNA isolation
S. aureus pre-treatment for plasmid preps
Solutions and Media Preparation
10x Lambda diluent (no gelatin)