Gill:M9 agar

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Materials

500 mL 1000 mL
Bacto Agar 7.5 g 15 g
dH2O 450 ml 880 ml
10 X M9 salts + glucose 50 ml 100 ml
1 M MgSO4 1 ml 2 ml (2 mM final)
1 M CaCl2 50 µl 0.1 ml (0.1 mM final)

Procedure

Autoclave agar/water solution, let cool. Aseptically add sterile salts + glucose, pour.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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