Gill:M9 agar

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: ==Materials== 500 mL: *Bacto agar 7.5 g *dH<sub>2</sub>O 450 ml *10 X M9 salts + glucose 50 ml *1 M MgSO<sub>4</sub> 1 ml (2 mM final) *1 M CaCl<sub>2</sub> 50 µl (0.1 mM final) 1000 ...)
Current revision (16:49, 23 April 2013) (view source)
 
Line 1: Line 1:
==Materials==
==Materials==
-
500 mL:
+
{| class="wikitable"
-
 
+
|-
-
*Bacto agar 7.5 g
+
!
-
*dH<sub>2</sub>O 450 ml
+
! 500 mL
-
*10 X M9 salts + glucose 50 ml
+
! 1000 mL
-
*1 M MgSO<sub>4</sub> 1 ml (2 mM final)
+
|-
-
*1 M CaCl<sub>2</sub> 50 µl (0.1 mM final)
+
| Bacto Agar
-
 
+
| 7.5 g
-
1000 mL:
+
| 15 g
-
 
+
|-
-
*Bacto agar 15 g
+
| dH<sub>2</sub>O  
-
*dH<sub>2</sub>O 880 ml
+
| 450 ml
-
*10 X M9 salts + glucose 100 ml
+
| 880 ml
-
*1 M MgSO<sub>4</sub> 2 ml (2 mM final)
+
|-
-
*1 M CaCl<sub>2</sub> 0.1 ml (0.1 mM final)
+
| 10 X M9 salts + glucose  
 +
| 50 ml
 +
| 100 ml
 +
|-
 +
| 1 M MgSO<sub>4</sub>
 +
| 1 ml
 +
| 2 ml
 +
| (2 mM final)
 +
|-
 +
| 1 M CaCl<sub>2</sub>  
 +
| 50 µl
 +
| 0.1 ml
 +
| (0.1 mM final)
 +
|}
==Procedure==
==Procedure==

Current revision

Materials

500 mL 1000 mL
Bacto Agar 7.5 g 15 g
dH2O 450 ml 880 ml
10 X M9 salts + glucose 50 ml 100 ml
1 M MgSO4 1 ml 2 ml (2 mM final)
1 M CaCl2 50 µl 0.1 ml (0.1 mM final)

Procedure

Autoclave agar/water solution, let cool. Aseptically add sterile salts + glucose, pour.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools