Gill:M9 agar: Difference between revisions

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(New page: ==Materials== 500 mL: *Bacto agar 7.5 g *dH<sub>2</sub>O 450 ml *10 X M9 salts + glucose 50 ml *1 M MgSO<sub>4</sub> 1 ml (2 mM final) *1 M CaCl<sub>2</sub> 50 µl (0.1 mM final) 1000 ...)
 
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==Materials==
==Materials==


500 mL:
{| class="wikitable"
 
|-
*Bacto agar 7.5 g
!
*dH<sub>2</sub>O 450 ml
! 500 mL
*10 X M9 salts + glucose 50 ml
! 1000 mL
*1 M MgSO<sub>4</sub> 1 ml (2 mM final)
|-
*1 M CaCl<sub>2</sub> 50 µl (0.1 mM final)
| Bacto Agar
 
| 7.5 g
1000 mL:
| 15 g
 
|-
*Bacto agar 15 g
| dH<sub>2</sub>O  
*dH<sub>2</sub>O 880 ml
| 450 ml
*10 X M9 salts + glucose 100 ml
| 880 ml
*1 M MgSO<sub>4</sub> 2 ml (2 mM final)
|-
*1 M CaCl<sub>2</sub> 0.1 ml (0.1 mM final)
| 10 X M9 salts + glucose  
| 50 ml
| 100 ml
|-
| 1 M MgSO<sub>4</sub>
| 1 ml
| 2 ml
| (2 mM final)
|-
| 1 M CaCl<sub>2</sub>  
| 50 µl
| 0.1 ml
| (0.1 mM final)
|}


==Procedure==
==Procedure==

Latest revision as of 13:49, 23 April 2013

Materials

500 mL 1000 mL
Bacto Agar 7.5 g 15 g
dH2O 450 ml 880 ml
10 X M9 salts + glucose 50 ml 100 ml
1 M MgSO4 1 ml 2 ml (2 mM final)
1 M CaCl2 50 µl 0.1 ml (0.1 mM final)

Procedure

Autoclave agar/water solution, let cool. Aseptically add sterile salts + glucose, pour.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!


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