Gill:M9 agar: Difference between revisions
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(New page: ==Materials== 500 mL: *Bacto agar 7.5 g *dH<sub>2</sub>O 450 ml *10 X M9 salts + glucose 50 ml *1 M MgSO<sub>4</sub> 1 ml (2 mM final) *1 M CaCl<sub>2</sub> 50 µl (0.1 mM final) 1000 ...) |
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==Materials== | ==Materials== | ||
500 mL | {| class="wikitable" | ||
|- | |||
! | |||
! 500 mL | |||
! 1000 mL | |||
|- | |||
| Bacto Agar | |||
| 7.5 g | |||
| 15 g | |||
|- | |||
| dH<sub>2</sub>O | |||
| 450 ml | |||
| 880 ml | |||
|- | |||
| 10 X M9 salts + glucose | |||
| 50 ml | |||
| 100 ml | |||
|- | |||
| 1 M MgSO<sub>4</sub> | |||
| 1 ml | |||
| 2 ml | |||
| (2 mM final) | |||
|- | |||
| 1 M CaCl<sub>2</sub> | |||
| 50 µl | |||
| 0.1 ml | |||
| (0.1 mM final) | |||
|} | |||
==Procedure== | ==Procedure== |
Latest revision as of 13:49, 23 April 2013
Materials
500 mL | 1000 mL | ||
---|---|---|---|
Bacto Agar | 7.5 g | 15 g | |
dH2O | 450 ml | 880 ml | |
10 X M9 salts + glucose | 50 ml | 100 ml | |
1 M MgSO4 | 1 ml | 2 ml | (2 mM final) |
1 M CaCl2 | 50 µl | 0.1 ml | (0.1 mM final) |
Procedure
Autoclave agar/water solution, let cool. Aseptically add sterile salts + glucose, pour.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!