Gill:Lambda diluent (SM buffer)

Materials

1000 mL:

• 1M Tris-HCl, pH 7.5, 50 ml (25 mM)
• NaCl 5.8 g (100 mM)
• MgSO4 1 g (8 mM)
• Gelatin (2% sol’n) 5 ml (0.01 %)
• dH₂O 475 ml

Procedure

Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!