Gill:Lambda diluent (SM buffer)

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
Current revision (16:02, 23 April 2013) (view source)
 
Line 11: Line 11:
|5.8 g (100 mM)
|5.8 g (100 mM)
|-
|-
-
|MgSO4
+
|MgSO<sub>4</sub>
|1 g (8 mM)
|1 g (8 mM)
|-
|-
Line 17: Line 17:
|5 ml (0.01 %)
|5 ml (0.01 %)
|-
|-
-
|dH2O
+
|dH<sub>2</sub>O
|475 ml
|475 ml
|}
|}

Current revision

Materials

1000 mL
1M Tris-HCl, pH 7.5 50 ml (25 mM)
NaCl 5.8 g (100 mM)
MgSO4 1 g (8 mM)
Gelatin (2% sol’n) 5 ml (0.01 %)
dH2O 475 ml

Procedure

Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools