Gill:Lambda diluent (SM buffer)

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==Materials==
==Materials==
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{| class="wikitable"
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1000 mL:
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|-
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!
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* 50 ml (25 mM) 1M Tris-HCl, pH 7.5
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! 1000 mL
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* 5.8 g (100 mM) NaCl
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|-
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* 1 g (8 mM) MgSO4
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|1M Tris-HCl, pH 7.5
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* 5 ml (0.01 %) Gelatin (2% sol’n)
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|50 ml (25 mM)
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* 475 ml dH<sub>2</sub>O
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|-
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|NaCl
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|5.8 g (100 mM)
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|-
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|MgSO4
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|1 g (8 mM)
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|-
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|Gelatin (2% sol’n)
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|5 ml (0.01 %)
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|-
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|dH2O
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|475 ml
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|}
==Procedure==
==Procedure==

Revision as of 16:01, 23 April 2013

Materials

1000 mL
1M Tris-HCl, pH 7.5 50 ml (25 mM)
NaCl 5.8 g (100 mM)
MgSO4 1 g (8 mM)
Gelatin (2% sol’n) 5 ml (0.01 %)
dH2O 475 ml

Procedure

Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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