Gill:Lambda diluent (SM buffer)

Materials

1000 mL:

• 50 ml (25 mM) 1M Tris-HCl, pH 7.5
• 5.8 g (100 mM) NaCl
• 1 g (8 mM) MgSO4
• 5 ml (0.01 %) Gelatin (2% sol’n)
• 475 ml dH₂O

Procedure

Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!