Gill:Lambda diluent (SM buffer): Difference between revisions
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|5.8 g (100 mM) | |5.8 g (100 mM) | ||
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| | |MgSO<sub>4</sub> | ||
|1 g (8 mM) | |1 g (8 mM) | ||
|- | |- | ||
| Line 17: | Line 17: | ||
|5 ml (0.01 %) | |5 ml (0.01 %) | ||
|- | |- | ||
| | |dH<sub>2</sub>O | ||
|475 ml | |475 ml | ||
|} | |} | ||
Latest revision as of 14:02, 23 April 2013
Materials
| 1000 mL | |
|---|---|
| 1M Tris-HCl, pH 7.5 | 50 ml (25 mM) |
| NaCl | 5.8 g (100 mM) |
| MgSO4 | 1 g (8 mM) |
| Gelatin (2% sol’n) | 5 ml (0.01 %) |
| dH2O | 475 ml |
Procedure
Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
