Gill:Lambda diluent (SM buffer): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: ==Materials== 1000 mL: * 1M Tris-HCl, pH 7.5, 50 ml (25 mM) * NaCl 5.8 g (100 mM) * MgSO4 1 g (8 mM) * Gelatin (2% sol’n) 5 ml (0.01 %) * dH<sub>2</sub>O 475 ml ==Procedure== Mix all ...) |
No edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Materials== | ==Materials== | ||
{| class="wikitable" | |||
1000 mL | |- | ||
! | |||
! 1000 mL | |||
|- | |||
|1M Tris-HCl, pH 7.5 | |||
|50 ml (25 mM) | |||
|- | |||
|NaCl | |||
|5.8 g (100 mM) | |||
|- | |||
|MgSO<sub>4</sub> | |||
|1 g (8 mM) | |||
|- | |||
|Gelatin (2% sol’n) | |||
|5 ml (0.01 %) | |||
|- | |||
|dH<sub>2</sub>O | |||
|475 ml | |||
|} | |||
==Procedure== | ==Procedure== | ||
Latest revision as of 14:02, 23 April 2013
Materials
| 1000 mL | |
|---|---|
| 1M Tris-HCl, pH 7.5 | 50 ml (25 mM) |
| NaCl | 5.8 g (100 mM) |
| MgSO4 | 1 g (8 mM) |
| Gelatin (2% sol’n) | 5 ml (0.01 %) |
| dH2O | 475 ml |
Procedure
Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
