- Bacto tryptone 10 g
- NaCl 10 g
- Yeast extract 5 g
- dH2O 1000 ml
Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!