Gill:LB broth

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Current revision (16:23, 7 November 2013) (view source)
 
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*10 g Bacto tryptone
*10 g Bacto tryptone
-
*10 g NaCl
+
*10 g NaCl (5 g NaCl for LB Lennox)
*5 g Yeast extract
*5 g Yeast extract
*1000 ml dH<sub>2</sub>O
*1000 ml dH<sub>2</sub>O

Current revision

Materials

1000 mL:

  • 10 g Bacto tryptone
  • 10 g NaCl (5 g NaCl for LB Lennox)
  • 5 g Yeast extract
  • 1000 ml dH2O

Procedure

Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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