Gill:LB broth: Difference between revisions
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*10 g Bacto tryptone | *10 g Bacto tryptone | ||
*10 g NaCl | *10 g NaCl (5 g NaCl for LB Lennox) | ||
*5 g Yeast extract | *5 g Yeast extract | ||
*1000 ml dH<sub>2</sub>O | *1000 ml dH<sub>2</sub>O |
Latest revision as of 14:23, 7 November 2013
Materials
1000 mL:
- 10 g Bacto tryptone
- 10 g NaCl (5 g NaCl for LB Lennox)
- 5 g Yeast extract
- 1000 ml dH2O
Procedure
Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!