Gill:LB broth

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(New page: ==Materials== 1000 mL: *Bacto tryptone 10 g *NaCl 10 g *Yeast extract 5 g *dH<sub>2</sub>O 1000 ml ==Procedure== Dissolve medium in water, then aliquot 100 ml into square milk bottles...)
Current revision (16:23, 7 November 2013) (view source)
 
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1000 mL:
1000 mL:
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*Bacto tryptone 10 g
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*10 g Bacto tryptone
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*NaCl 10 g
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*10 g NaCl (5 g NaCl for LB Lennox)
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*Yeast extract 5 g
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*5 g Yeast extract
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*dH<sub>2</sub>O 1000 ml
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*1000 ml dH<sub>2</sub>O
==Procedure==
==Procedure==

Current revision

Materials

1000 mL:

  • 10 g Bacto tryptone
  • 10 g NaCl (5 g NaCl for LB Lennox)
  • 5 g Yeast extract
  • 1000 ml dH2O

Procedure

Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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