Gill:Back Door

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===Using the Autoclave===
===Using the Autoclave===
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* Do not change the temperature on the autoclaves in the Biochemistry Building.
 
* Use autoclave gloves when loading/unloading the autoclave. A face shield can also be used.
* Use autoclave gloves when loading/unloading the autoclave. A face shield can also be used.
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* Make sure the pressure is at zero before opening.
* Make sure the pressure is at zero before opening.
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* Fill containers to under 75% capacity.
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* Do not change the temperature on the autoclaves in the Biochemistry Building.
====Liquids====
====Liquids====

Revision as of 16:06, 24 April 2013

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Contents

Safety

Disposing of Waste

Biohazardous Waste

Solid biohazardous waste should be autoclaved in the kill autoclaves in the Biochemistry Building at 121 °C for an hour. Close the plastic waste bag, leaving a small opening at the top so that steam can enter and escape. Place the bag in an autoclave-safe tray, and place heat-sensitive tape over the biohazard symbol. After autoclaving, allow the bag to cool and then dispose of it in the dumpster.

To dispose of liquid biohazardous waste, add 10% bleach to the flask and let it sit in the sink for a while, then dump the waste down the drain. Wash the flask and place it on the drying rack.

Chemical Waste

Cleaning Spills

To clean up a biological spill, cover the spill with paper towels and add undiluted bleach. Let it sit, and then dispose of the paper towels in a biohazard waste bin.

Using the Autoclave

  • Use autoclave gloves when loading/unloading the autoclave. A face shield can also be used.
  • Loosen all caps before autoclaving, and tape them on one side.
  • Make sure the pressure is at zero before opening.
  • Fill containers to under 75% capacity.
  • Do not change the temperature on the autoclaves in the Biochemistry Building.

Liquids

Autoclave for 30 minutes using the liquid cycle. After autoclaving, crack the door to release steam and then slowly open the door. Allow the liquids to cool slightly before removing the tray.

Solids

Autoclave for 30 minutes using the gravity cycle, then move to the dryer overnight.

Do Not Autoclave...

  • Hazardous chemical waste. E.g. bleach, ethidium bromide, HCl, chloroform...
  • Non-heat-resistant plastics. E.g. polystyrene, polyethylene, high density polyethylene...
  • Radioactive waste.
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