Gill:50X Vogel-Bonner salts

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Revision as of 13:41, 23 April 2013 by Jacqueline K. Grimm (talk | contribs) (New page: ==Materials== {| class="wikitable" |- ! ! 1000 mL |- |Warm dd H2O (45°C) |670 ml |- |MgSO4 • 7 H2O |10 g |- |Citric acid monohydrate |100 g |- |K2HPO4 |500 g |- ...)
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Materials

1000 mL
Warm dd H2O (45°C) 670 ml
MgSO4 • 7 H2O 10 g
Citric acid monohydrate 100 g
K2HPO4 500 g
NaHNH4PO4 • 4H2O 175 g

Procedure

Add salts in the order indicated to warm water in a 2-liter flask placed on magnetic stirring hot plate. Allow each salt to dissolve completely before adding the next. Adjust the volume to 1 liter. Distribute into two 1-liter glass bottles. Autoclave for 20 min at 121°C, store at RT.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!


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