Gill:10 X M9 salts + glucose

From OpenWetWare

Revision as of 17:11, 23 April 2013 by Jacqueline K. Grimm (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Materials

1000 mL:

  • 700 ml dH2O
  • 128 g Na2HPO4 • 7H2O
  • 30 g KH2PO4
  • 5 g NaCl
  • 10 g NH4Cl
  • 40 g Glucose

Procedure

Add all components to water, dissolve, adjust to 1000 ml. Filter sterilize at 0.22 µm (do not autoclave), store at RT. If using anhydrous Na2HPO4, multiply the weight for this component by 0.53.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools