Gill:10 X M9 salts

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: ==Materials== 1000 mL: *dH<sub>2</sub>O 700 ml *Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O 128 g *KH<sub>2</sub>PO<sub>4</sub> 30 g *NaCl 5 g *NH<sub>4</sub>Cl 10 g ==Procedure==...)
Current revision (16:10, 23 April 2013) (view source)
m
 
Line 3: Line 3:
1000 mL:
1000 mL:
-
*dH<sub>2</sub>O 700 ml
+
*700 ml dH<sub>2</sub>O
-
*Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O 128 g
+
*128 g Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O
-
*KH<sub>2</sub>PO<sub>4</sub> 30 g
+
*30 g KH<sub>2</sub>PO<sub>4</sub>
-
*NaCl 5 g
+
*5 g NaCl
-
*NH<sub>4</sub>Cl 10 g
+
*10 g NH<sub>4</sub>Cl
==Procedure==
==Procedure==

Current revision

Materials

1000 mL:

  • 700 ml dH2O
  • 128 g Na2HPO4 • 7H2O
  • 30 g KH2PO4
  • 5 g NaCl
  • 10 g NH4Cl

Procedure

Add salts to water, dissolve, adjust to 1000 ml. Autoclave, store at RT. If using anhydrous Na2HPO4, multiply the weight for this component by 0.53.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools