Gill:10 X M9 salts: Difference between revisions
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(New page: ==Materials== 1000 mL: *dH<sub>2</sub>O 700 ml *Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O 128 g *KH<sub>2</sub>PO<sub>4</sub> 30 g *NaCl 5 g *NH<sub>4</sub>Cl 10 g ==Procedure==...) |
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| Line 3: | Line 3: | ||
1000 mL: | 1000 mL: | ||
*dH<sub>2</sub>O | *700 ml dH<sub>2</sub>O | ||
*Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O | *128 g Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O | ||
*KH<sub>2</sub>PO<sub>4</sub> | *30 g KH<sub>2</sub>PO<sub>4</sub> | ||
* | *5 g NaCl | ||
*NH<sub>4</sub>Cl | *10 g NH<sub>4</sub>Cl | ||
==Procedure== | ==Procedure== | ||
Latest revision as of 14:10, 23 April 2013
Materials
1000 mL:
- 700 ml dH2O
- 128 g Na2HPO4 • 7H2O
- 30 g KH2PO4
- 5 g NaCl
- 10 g NH4Cl
Procedure
Add salts to water, dissolve, adjust to 1000 ml. Autoclave, store at RT. If using anhydrous Na2HPO4, multiply the weight for this component by 0.53.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
