Gentamicin - Revision history

Skosuri at 19:33, 22 July 2008

Skosuri — Tue, 22 Jul 2008 19:33:14 GMT

←Older revision		Revision as of 19:33, 22 July 2008
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	===Resistance Gene===		===Resistance Gene===
-	The most common resistance gene is the aacA-aphD gene found in the transposon Tn4001. It conveys resistance to both ~~Gentamycin~~ and Kanamycin in two separate domains. Another common resistance gene is the aacC1 gene, (conveying resistance to ~~Gentamycin~~ only) is found in the plasmid pJN105. <cite>Newman-Gene-1999, Wohlleben-MolGenGenet-1989</cite>	+	The most common resistance gene is the aacA-aphD gene found in the transposon Tn4001. It conveys resistance to both Gentamicin and Kanamycin in two separate domains. Another common resistance gene is the aacC1 gene, (conveying resistance to Gentamicin only) is found in the plasmid pJN105. <cite>Newman-Gene-1999, Wohlleben-MolGenGenet-1989</cite>

	===Safety===		===Safety===

Skosuri at 19:30, 22 July 2008

Skosuri — Tue, 22 Jul 2008 19:30:27 GMT

←Older revision		Revision as of 19:30, 22 July 2008
Line 23:		Line 23:

	===Safety===		===Safety===
-	~~Gentamycin~~ is an important antibiotic for human use. Think about alternative antibiotics when choosing to incorporate ~~gentamycin~~ resistance into your constructs. Particularly, think carefully about inserting ~~gentamycin~~ resistance into mobile plasmids or mobile transposons.	+	Gentamicin is an important antibiotic for human use. Think about alternative antibiotics when choosing to incorporate gentamicin resistance into your constructs. Particularly, think carefully about inserting gentamicin resistance into mobile plasmids or mobile transposons.

	==References==		==References==

Reshma P. Shetty at 17:46, 26 July 2007

Reshma P. Shetty — Thu, 26 Jul 2007 17:46:39 GMT

←Older revision		Revision as of 17:46, 26 July 2007
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	#Wohlleben-MolGenGenet-1989 pmid=2549372		#Wohlleben-MolGenGenet-1989 pmid=2549372
	</biblio>		</biblio>
		+
		+	[[Category:Material]] [[Category:Antibiotic]]

Austin J. Che at 19:28, 27 April 2007

Austin J. Che — Fri, 27 Apr 2007 19:28:14 GMT

←Older revision		Revision as of 19:28, 27 April 2007
Line 21:		Line 21:
	===Resistance Gene===		===Resistance Gene===
	The most common resistance gene is the aacA-aphD gene found in the transposon Tn4001. It conveys resistance to both Gentamycin and Kanamycin in two separate domains. Another common resistance gene is the aacC1 gene, (conveying resistance to Gentamycin only) is found in the plasmid pJN105. <cite>Newman-Gene-1999, Wohlleben-MolGenGenet-1989</cite>		The most common resistance gene is the aacA-aphD gene found in the transposon Tn4001. It conveys resistance to both Gentamycin and Kanamycin in two separate domains. Another common resistance gene is the aacC1 gene, (conveying resistance to Gentamycin only) is found in the plasmid pJN105. <cite>Newman-Gene-1999, Wohlleben-MolGenGenet-1989</cite>
-
-	~~===Plate color code===~~
-	~~Purple~~

	===Safety===		===Safety===

Jakob Suckale: article content moved from misspelled entry gentamYcin

Jakob Suckale — Fri, 04 Aug 2006 13:57:47 GMT

article content moved from misspelled entry gentamYcin

New page

==Experimental==

===Stock Solution===
10mg/ml in H<sub>2</sub>0, store at -20°C (from [http://molbiol.edu.ru/eng/protocol/01_05.html here])

Soluble in water at least to 200 mg/ml

Insoluble in 50% alcohol

===Working Concentration===
20μg/ml <cite>Khlebnikov-JIndMicrobiolBiotechnol-2002</cite>

80μg/ml for Mycoplasma selection

===Mode of Action===

Inhibits protein synthesis by binding to L6 protein of the 50S ribosomal subunit.

(from [[Molecular Cloning]])

===Resistance Gene===
The most common resistance gene is the aacA-aphD gene found in the transposon Tn4001. It conveys resistance to both Gentamycin and Kanamycin in two separate domains. Another common resistance gene is the aacC1 gene, (conveying resistance to Gentamycin only) is found in the plasmid pJN105. <cite>Newman-Gene-1999, Wohlleben-MolGenGenet-1989</cite>

===Plate color code===
Purple

===Safety===
Gentamycin is an important antibiotic for human use. Think about alternative antibiotics when choosing to incorporate gentamycin resistance into your constructs. Particularly, think carefully about inserting gentamycin resistance into mobile plasmids or mobile transposons.

==References==
<biblio>
#Khlebnikov-JIndMicrobiolBiotechnol-2002 pmid=12080425
#Newman-Gene-1999 pmid=10023058
#Wohlleben-MolGenGenet-1989 pmid=2549372
</biblio>