Genotypes

This information should be verified further. It was copied and pasted and edited by hand and so formatting/human errors may have been introduced. If sources are not cited, it is because this list was compiled prior to the wiki and the information source wasn't recorded.

Commonly used strains

DH5α

F^-, Φ80dlacZΔM15Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(r_K^- m_K⁺), supE44, λ–, thi-1, gyrA96, relA1

• From a GIBCO BRL list of competent cells.
• Another source also mentioned phoA
• Reference: FOCUS (1986) 8:2, 9.

DB3.1

F- gyrA462 endA1 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB-) supE44 ara14 galK2 lacY1proA2 rpsL20(Smr) xyl5 Δleu mtl1

D1210

HB101 lacI^q, lacY⁺

JM109

F^', traD36, proA⁺B⁺, lacI^q Δ(lacZ)M15/ Δ(lac-proAB), glnV44, e14-, gyrA96, recA1, relA1, endA1, thi, hsdR17

• From NEB
• Partly restriction-deficient; good strain for cloning repetitive DNA (RecA^–).
• Suppresses many amber mutations when glutamine is acceptable but not the S₁₀₀ or S₇ mutations of λ, e.g., λgt11.
• Can also be used for M13 cloning/sequencing and blue/white screening.

BW26434, CGSC Strain # 7658

Δ(araD-araB)567, Δ(lacA-lacZ)514(::kan), lacIp-4000(lacI^Q), λ^-, rpoS396(Am)?, rph-1, Δ(rhaD-rhaB)568, hsdR514

• This information is from a printout sent by the E. coli Genetic Stock Center with the strain.
• B.L. Wanner strain
• rph-1 is a 1bp deletion that results in a frameshift over last 15 codons and has a polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium. (Jensen 1993 J Bact. 175:3401)
• Δ(araD-araB)567 was formerly called ΔaraBAD_AH33 by Datsenko and Wanner
• Am = amber(UAG) mutation
• Reference: Datsenko and Wanner, 2000, PNAS, 97:6640

Miscellaneous strains

HB101

Please note that different sources have different genotypes so treat this information with caution.
supE44, Δ(mcrC-mrr), recA13, ara-14, proA2, lacY1, galK2, rpsL20, xyl-5, mtl-1, leuB6, thi-1
F^-, mcrB, mrr, hsdS20(r_B^- m_B^-), recA13, leuB6, ara-14, proA2, lacY1, galK2, xyl-5, mtl-1, rpsL20(Sm^r), supE44, λ^-

• From a GIBCO BRL list of competent cells.

BL21 (DE3)

F-ompT hsdS B(r_B^- m_B^-) gal dcm (DE3)

BL21(DE3) (Novagen)

17 F– ompT gal [dcm] [lon] hsdSB (r_B^- m_B^-; an E. coli B strain) with DE3, a λ prophage carrying the T7 RNA polymerase gene

BL21 (DE3)pLysS

F-ompT hsdS B(r_B^- m_B^-)gal dcm (DE3) pLysS (cam R)

XL1-Blue (Stratagene)

15 F' ::Tn 10 proA+ B+ lacI^q, Δ(lacZ)M15/recA1, endA1, gyrA96, (Nalr), thi, hsdR17(r_K^- m_K⁺), glnV44, relA1, lac