Generating antibodies: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(external links) |
|||
| Line 15: | Line 15: | ||
== External links == | == External links == | ||
* [http://www.hopkins-arthritis.org/physician-corner/education/mono_anti.html Introduction to monoclonal antibody generation] by Mark Soloski, Johns Hopkins | * [http://www.hopkins-arthritis.org/physician-corner/education/mono_anti.html Introduction to monoclonal antibody generation] by Mark Soloski, Johns Hopkins | ||
* [http://mabs.unc.edu/services.htm Hybridoma and in vitro generation of antibodies], Antibody Facility, UNC Chapel Hill | |||
Protocol Online (methods forum) | |||
* [http://www.protocol-online.org/cgi-bin/htsearch?config=htdig_pol&restrict=&exclude=&words=antibody Protocol-online.org archive, keyword antibody] and [http://www.protocol-online.org/biology-forums/antibody.html discussions indexed under antibody] | |||
* [http://www.protocol-online.org/biology-forums/posts/29480.html Affinity purification] | |||
* [] | |||
* [] | |||
* [] | |||
Wikipedia | Wikipedia | ||
Revision as of 08:13, 22 April 2008
You are hot in pursuit of an interesting protein but there are no commercial antibodies or they are bad. It's time to think about raising your own antibodies against the protein. It will be a time and money consuming undertaking but once you developed a specific antibody or set of antibodies, you will be in a unique position to generate interesting data.
Early decisions
Type of antibody
Source organism
Type of antigen to use
See also
External links
- Introduction to monoclonal antibody generation by Mark Soloski, Johns Hopkins
- Hybridoma and in vitro generation of antibodies, Antibody Facility, UNC Chapel Hill
Protocol Online (methods forum)
- Protocol-online.org archive, keyword antibody and discussions indexed under antibody
- Affinity purification
- []
- []
- []
Wikipedia
