GYT

From OpenWetWare

Revision as of 10:58, 22 July 2008 by Andrew Ang (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Summary

SOB Medium. Used in growing bacteria for preparing electrocompetent cells

Ingredients

  • 10% (v/v) glycerol
  • 0.125% (w/v) yeast extract
  • .25% (w/v) tryptone

Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Sore in 2.5-ml aliquots at 4°C.

From Molecular Cloning

Personal tools