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SOB Medium. Used in growing bacteria for preparing electrocompetent cells


  • 10% (v/v) glycerol
  • 0.125% (w/v) yeast extract
  • .25% (w/v) tryptone

Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Sore in 2.5-ml aliquots at 4°C.

From Molecular Cloning

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