GYT

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(Summary)
Current revision (12:53, 22 July 2008) (view source)
(Ingredients)
 
Line 7: Line 7:
*.25% (w/v) tryptone
*.25% (w/v) tryptone
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Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Sore in 2.5-ml aliquots at 4°C.  
+
Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Store in 2.5-ml aliquots at 4°C.  
From ''Molecular Cloning''
From ''Molecular Cloning''

Current revision

Summary

GYT Medium. Used in preparing electrocompetent cells.

Ingredients

  • 10% (v/v) glycerol
  • 0.125% (w/v) yeast extract
  • .25% (w/v) tryptone

Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Store in 2.5-ml aliquots at 4°C.

From Molecular Cloning

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