GYT: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Andrew Ang (talk | contribs) |
Andrew Ang (talk | contribs) |
||
| Line 7: | Line 7: | ||
*.25% (w/v) tryptone | *.25% (w/v) tryptone | ||
Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. | Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Store in 2.5-ml aliquots at 4°C. | ||
From ''Molecular Cloning'' | From ''Molecular Cloning'' | ||
Latest revision as of 10:53, 22 July 2008
Summary
GYT Medium. Used in preparing electrocompetent cells.
Ingredients
- 10% (v/v) glycerol
- 0.125% (w/v) yeast extract
- .25% (w/v) tryptone
Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Store in 2.5-ml aliquots at 4°C.
From Molecular Cloning
