GYT

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(New page: == Summary == SOB Medium. Used in growing bacteria for preparing electrocompetent cells == Ingredients == *10% (v/v) glycerol *0.125% (w/v) yeast extract *.25% (w/v) tryptone Sterilize ...)
Current revision (12:53, 22 July 2008) (view source)
(Ingredients)
 
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== Summary ==
== Summary ==
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SOB Medium.  Used in growing bacteria for preparing electrocompetent cells
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GYT Medium.  Used in preparing electrocompetent cells.
== Ingredients ==
== Ingredients ==
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*.25% (w/v) tryptone
*.25% (w/v) tryptone
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Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Sore in 2.5-ml aliquots at 4°C.  
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Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Store in 2.5-ml aliquots at 4°C.  
From ''Molecular Cloning''
From ''Molecular Cloning''

Current revision

Summary

GYT Medium. Used in preparing electrocompetent cells.

Ingredients

  • 10% (v/v) glycerol
  • 0.125% (w/v) yeast extract
  • .25% (w/v) tryptone

Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Store in 2.5-ml aliquots at 4°C.

From Molecular Cloning

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