GYT
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(New page: == Summary == SOB Medium. Used in growing bacteria for preparing electrocompetent cells == Ingredients == *10% (v/v) glycerol *0.125% (w/v) yeast extract *.25% (w/v) tryptone Sterilize ...) |
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Revision as of 13:00, 22 July 2008
Summary
GYT Medium. Used in preparing electrocompetent cells
Ingredients
- 10% (v/v) glycerol
- 0.125% (w/v) yeast extract
- .25% (w/v) tryptone
Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Sore in 2.5-ml aliquots at 4°C.
From Molecular Cloning
