GFP

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The fluorophore is tyrosine Y66 and the surrounding amino acids of Ser(65)-Tyr(66)-Gly(67) are also critical [1].

GFP Variants

  • GFPmut3b - S65G, S72A
  • GFPmut3* - mutations from wt: S2R,S65G,S72A
    • This is GFPmut3b[2] with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.[3]
  • Emerald
  • EGFP F64L, S65T + optimized for human codons with 35-fold increase in fluorescence over GFP [1]

References

  1. Li X, Zhang G, Ngo N, Zhao X, Kain SR, and Huang CC. . pmid:9353317. PubMed HubMed [li97]
  2. Cormack BP, Valdivia RH, and Falkow S. . pmid:8707053. PubMed HubMed [Cormack]
  3. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, and Molin S. . pmid:9603842. PubMed HubMed [Andersen]
All Medline abstracts: PubMed HubMed

Molecular Biology and Muation of Green Fluorescent Protein (Book Chapter, Zacharias & Tsien)

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