GFP: Difference between revisions
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==GFP Variants== | ==GFP Variants== | ||
*GFPmut3b - S65G, S72A | |||
*[http://parts.mit.edu/registry/index.php/Part:BBa_E0040 ''GFP''mut3*] - mutations from wt: S2R,S65G,S72A | *[http://parts.mit.edu/registry/index.php/Part:BBa_E0040 ''GFP''mut3*] - mutations from wt: S2R,S65G,S72A | ||
**This is GFPmut3b<cite>Cormack</cite> with a degredation tag attached. | **This is GFPmut3b<cite>Cormack</cite> with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors. | ||
==References== | ==References== | ||
Revision as of 20:55, 30 May 2006
GFP Variants
- GFPmut3b - S65G, S72A
- GFPmut3* - mutations from wt: S2R,S65G,S72A
- This is GFPmut3b[1] with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.
References
- Cormack BP, Valdivia RH, and Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 1996;173(1 Spec No):33-8. DOI:10.1016/0378-1119(95)00685-0 |
Molecular Biology and Muation of Green Fluorescent Protein (Book Chapter, Zacharias & Tsien)
