GFP
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*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&val=1169893 wtGFP on NCBI] | *[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&val=1169893 wtGFP on NCBI] | ||
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| + | The fluorophore is tyrosine Y66 and the surrounding amino acids of Ser(65)-Tyr(66)-Gly(67) are also critical <cite>li97</cite>. | ||
==GFP Variants== | ==GFP Variants== | ||
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**This is GFPmut3b<cite>Cormack</cite> with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.<cite>Anderson</cite> | **This is GFPmut3b<cite>Cormack</cite> with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.<cite>Anderson</cite> | ||
*[[Emerald]] | *[[Emerald]] | ||
| + | * EGFP F64L, S65T + optimized for human codons <cite>li97</cite> | ||
==References== | ==References== | ||
<biblio> | <biblio> | ||
| + | #li97 pmid=9353317 | ||
#Cormack pmid=8707053 | #Cormack pmid=8707053 | ||
#Andersen pmid=9603842 | #Andersen pmid=9603842 | ||
Revision as of 14:45, 27 September 2006
The fluorophore is tyrosine Y66 and the surrounding amino acids of Ser(65)-Tyr(66)-Gly(67) are also critical [1].
GFP Variants
- GFPmut3b - S65G, S72A
- GFPmut3* - mutations from wt: S2R,S65G,S72A
- Emerald
- EGFP F64L, S65T + optimized for human codons [1]
References
- Li X, Zhang G, Ngo N, Zhao X, Kain SR, and Huang CC. . pmid:9353317.
- Cormack BP, Valdivia RH, and Falkow S. . pmid:8707053.
- Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, and Molin S. . pmid:9603842.
Molecular Biology and Muation of Green Fluorescent Protein (Book Chapter, Zacharias & Tsien)
