G418 - Revision history

Reshma P. Shetty at 17:46, 26 July 2007

Reshma P. Shetty — Thu, 26 Jul 2007 17:46:16 GMT

←Older revision		Revision as of 17:46, 26 July 2007
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	* FAQ on G418 [http://www.g418.com/faq.htm]		* FAQ on G418 [http://www.g418.com/faq.htm]
	* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]		* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]
		+
		+	[[Category:Material]] [[Category:Antibiotic]]

Jakob Suckale: aminoglycoside link

Jakob Suckale — Fri, 04 Aug 2006 13:56:48 GMT

aminoglycoside link

←Older revision		Revision as of 13:56, 4 August 2006
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-	Aminoglycoside antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).	+	[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).

	==Experimental==		==Experimental==

Jakob Suckale: lead added + G418 = geneticin

Jakob Suckale — Fri, 04 Aug 2006 13:47:45 GMT

lead added + G418 = geneticin

←Older revision		Revision as of 13:47, 4 August 2006
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		+	Aminoglycoside antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).
		+
	==Experimental==		==Experimental==

Jakob Suckale: /* Links */

Jakob Suckale — Fri, 04 Aug 2006 13:20:45 GMT

Links

←Older revision		Revision as of 13:20, 4 August 2006
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	==Links==		==Links==
-	* related antibiotics: [[kanamycin]], [[neomycin]]	+	* related antibiotics: [[kanamycin]], [[neomycin]] (all aminoglycosides)
	* FAQ on G418 [http://www.g418.com/faq.htm]		* FAQ on G418 [http://www.g418.com/faq.htm]
	* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]		* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]

Jakob Suckale: /* Links */ related antibiotics

Jakob Suckale — Fri, 04 Aug 2006 13:14:36 GMT

Links: related antibiotics

←Older revision		Revision as of 13:14, 4 August 2006
Line 20:		Line 20:

	==Links==		==Links==
		+	* related antibiotics: [[kanamycin]], [[neomycin]]
	* FAQ on G418 [http://www.g418.com/faq.htm]		* FAQ on G418 [http://www.g418.com/faq.htm]
	* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]		* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]

Jakob Suckale: less tiny headings for better page view

Jakob Suckale — Fri, 04 Aug 2006 13:13:41 GMT

less tiny headings for better page view

←Older revision		Revision as of 13:13, 4 August 2006
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	==Experimental==		==Experimental==
-	~~Gentamycin analog, used to select for the KanMX marker in yeast.~~

-	~~===Stock Solution===~~	+	* '''stock solution''': 200mg/mL (1000X), stored at -20 C
-	200mg/mL (1000X), stored at -20 C	+	* '''working concentration''': working concentration depends on organism and purpose of antibiotic application; stable at RT

-	~~===Working Concentration===~~	+	:* bacteria selection: 8-16 µg/ml
-	~~Working concentration depends on organism and purpose of antibiotic application.~~	+

-	* ~~bacteria~~ selection: 8-16 µg/ml	+	:* plant cell maintenance: 10 µg/ml
		+	:* plant cell selection: 25-50 µg/ml

-	* ~~plant~~ cell maintenance: 10 µg/ml	+	:* mammalian cell maintenance: 200 µg/ml
-	* ~~plant~~ cell selection: 25-50 µg/ml	+	:* mammalian cell selection: 400 µg/ml (range: 300-1000 µg/ml)
		+	:* yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L (=µg/ml) G418.

-	* mammalian cell maintenance: 200 µg/ml	+	==Description==
-	* mammalian cell selection: 400 µg/ml	+
-	* yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L (=~~µg/ml) G418.~~	+

-	~~==Mode of Action==~~	+	* Gentamycin analog, used to select for the KanMX marker in yeast.
-		+	* '''mode of action''': irreversibly binds to 80S ribosomal subunit, disrupting proofreading.
-	~~Irreversibly~~ binds to 80S ribosomal subunit, disrupting proofreading.	+	* '''mode of resistance''': ''neo/kan'' gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, [[neomycin]] and [[kanamycin]] by phosphorylation.
-		+
-	~~==Mode~~ of resistance==	+
-		+
-	''neo/kan'' gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, [[neomycin]] and [[kanamycin]] by phosphorylation.	+
-		+
-	~~==Safety==~~	+

	==Links==		==Links==
	* FAQ on G418 [http://www.g418.com/faq.htm]		* FAQ on G418 [http://www.g418.com/faq.htm]
		+	* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]

Jakob Suckale: /* Mode of Action */ removed duplicate info

Jakob Suckale — Fri, 04 Aug 2006 12:51:37 GMT

Mode of Action: removed duplicate info

←Older revision		Revision as of 12:51, 4 August 2006
Line 19:		Line 19:
	==Mode of Action==		==Mode of Action==

-	Irreversibly binds to 80S ribosomal subunit, disrupting proofreading~~. Can be blocked by aminoglycoside-3'-phosphotransferase~~.	+	Irreversibly binds to 80S ribosomal subunit, disrupting proofreading.

	==Mode of resistance==		==Mode of resistance==

Jakob Suckale: resistance info added, links to neo & kan

Jakob Suckale — Fri, 04 Aug 2006 12:51:00 GMT

resistance info added, links to neo & kan

←Older revision		Revision as of 12:51, 4 August 2006
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	* mammalian cell selection: 400 µg/ml		* mammalian cell selection: 400 µg/ml
	* yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L (=µg/ml) G418.		* yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L (=µg/ml) G418.
-

	==Mode of Action==		==Mode of Action==
-		+
	Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.		Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.
		+
		+	==Mode of resistance==
		+
		+	''neo/kan'' gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, [[neomycin]] and [[kanamycin]] by phosphorylation.

	==Safety==		==Safety==

Jakob Suckale: working concentrations by organism

Jakob Suckale — Fri, 04 Aug 2006 12:47:27 GMT

working concentrations by organism

←Older revision		Revision as of 12:47, 4 August 2006
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	===Working Concentration===		===Working Concentration===
-	~~After~~ transformation incubate culture at ~~30 C~~ for 2-3 hours and then plate on [[YPD]] containing 200 mg/L G418.	+	Working concentration depends on organism and purpose of antibiotic application.
		+
		+	* bacteria selection: 8-16 µg/ml
		+
		+	* plant cell maintenance: 10 µg/ml
		+	* plant cell selection: 25-50 µg/ml
		+
		+	* mammalian cell maintenance: 200 µg/ml
		+	* mammalian cell selection: 400 µg/ml
		+	* yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L (=µg/ml) G418.
		+

	==Mode of Action==		==Mode of Action==
		+
	Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.		Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.

	==Safety==		==Safety==
		+
		+	==Links==
		+	* FAQ on G418 [http://www.g418.com/faq.htm]

Josh K. Michener at 16:52, 1 August 2005

Josh K. Michener — Mon, 01 Aug 2005 16:52:09 GMT

←Older revision		Revision as of 16:52, 1 August 2005
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-	~~Used~~ to select for the KanMX marker in yeast.	+	==Experimental==
		+	Gentamycin analog, used to select for the KanMX marker in yeast.

		+	===Stock Solution===
		+	200mg/mL (1000X), stored at -20 C
		+
		+	===Working Concentration===
	After transformation incubate culture at 30 C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L G418.		After transformation incubate culture at 30 C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L G418.

-	~~The stock solution is 200mg/mL (1000X) and is stored at~~ -~~20 C~~	+	==Mode of Action==
		+	Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.
		+
		+	==Safety==