G418

Experimental

Gentamycin analog, used to select for the KanMX marker in yeast.

Stock Solution

200mg/mL (1000X), stored at -20 C

Working Concentration

After transformation incubate culture at 30 C for 2-3 hours and then plate on YPD containing 200 mg/L G418.

Mode of Action

Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.