G418: Difference between revisions
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===Working Concentration=== | ===Working Concentration=== | ||
Working concentration depends on organism and purpose of antibiotic application. | |||
* bacteria selection: 8-16 µg/ml | |||
* plant cell maintenance: 10 µg/ml | |||
* plant cell selection: 25-50 µg/ml | |||
* mammalian cell maintenance: 200 µg/ml | |||
* mammalian cell selection: 400 µg/ml | |||
* yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L (=µg/ml) G418. | |||
==Mode of Action== | ==Mode of Action== | ||
Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase. | Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase. | ||
==Safety== | ==Safety== | ||
==Links== | |||
* FAQ on G418 [http://www.g418.com/faq.htm] | |||
Revision as of 05:47, 4 August 2006
Experimental
Gentamycin analog, used to select for the KanMX marker in yeast.
Stock Solution
200mg/mL (1000X), stored at -20 C
Working Concentration
Working concentration depends on organism and purpose of antibiotic application.
- bacteria selection: 8-16 µg/ml
- plant cell maintenance: 10 µg/ml
- plant cell selection: 25-50 µg/ml
- mammalian cell maintenance: 200 µg/ml
- mammalian cell selection: 400 µg/ml
- yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on YPD containing 200 mg/L (=µg/ml) G418.
Mode of Action
Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.
Safety
Links
- FAQ on G418 [1]
