G418: Difference between revisions
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==Experimental== | |||
Gentamycin analog, used to select for the KanMX marker in yeast. | |||
===Stock Solution=== | |||
200mg/mL (1000X), stored at -20 C | |||
===Working Concentration=== | |||
After transformation incubate culture at 30 C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L G418. | After transformation incubate culture at 30 C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L G418. | ||
==Mode of Action== | |||
Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase. | |||
==Safety== | |||
Revision as of 09:52, 1 August 2005
Experimental
Gentamycin analog, used to select for the KanMX marker in yeast.
Stock Solution
200mg/mL (1000X), stored at -20 C
Working Concentration
After transformation incubate culture at 30 C for 2-3 hours and then plate on YPD containing 200 mg/L G418.
Mode of Action
Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.
