G418

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Used to select for the KanMX marker in yeast.
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==Experimental==
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Gentamycin analog, used to select for the KanMX marker in yeast.
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===Stock Solution===
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200mg/mL (1000X), stored at -20 C
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===Working Concentration===
After transformation incubate culture at 30 C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L G418.
After transformation incubate culture at 30 C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L G418.
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The stock solution is 200mg/mL (1000X) and is stored at -20 C
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==Mode of Action==
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Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.
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==Safety==

Revision as of 12:52, 1 August 2005

Contents

Experimental

Gentamycin analog, used to select for the KanMX marker in yeast.

Stock Solution

200mg/mL (1000X), stored at -20 C

Working Concentration

After transformation incubate culture at 30 C for 2-3 hours and then plate on YPD containing 200 mg/L G418.

Mode of Action

Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.

Safety

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