G.tigrina Hox gene DthoxC insertion into prokaryote E.coli / (by UNIamCloning)/2011/11/16: Difference between revisions
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(Autocreate 2011/11/16 Entry for G.tigrina_Hox_gene_DthoxC_insertion_into_prokaryote_E.coli_/_(by_UNIamCloning)) |
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== | ==Restriction Digest and Purification of Plasmid== | ||
* | * Today we ran a restriction digest of a blank vector using Ecor1 and Pst1 restriction enzymes, 10X buffer, and plasmid DNA. After the digest, we purified the DNA using the PCR Purification Kit. This allowed for the DNA to be washed clean of the enzymes. Tomorrow, we will run a gel of the DNA to check our results (mainly to see if we still have DNA and if it was at at the right band length). Also, we will use the digested samples of ATF1, GFP, and the blank vector and ligate them together. | ||
Revision as of 15:14, 16 November 2011
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Restriction Digest and Purification of Plasmid
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