French Lab: Difference between revisions
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Version: 15 October 2015 | |||
===Projects=== | ===Current Projects=== | ||
* | *Combinatorial assembly of gene cassettes for cellulose degradation: Dr. Chao-Kuo Liu | ||
* | *Conversion of green algal polysaccharides to biofuels: Alejandro Salinas-Vaccaro | ||
* | *Enzyme engineering for cellulose degradation: Kwabena Duedu | ||
* | *Artifical consortia for conversion of biomass to useful products: Craig Munns | ||
* | *Arsenic Biosensor Consortium: Dr. David Radford | ||
* | *Novel biosensor formats: Jan Oltmanns | ||
* | *Biosensors for use in developing nations: Chris Nwanko | ||
* | *Novel approaches to radiotherapy using engineered proteins: Dr. Jon Stanley | ||
*Markerless genome editing in E. coli and related bacteria: Kuan Meng (Evan) Lee | |||
===Recent Projects=== | |||
*Novel methods for DNA assembly: PaperClip: Dr. Maryia Trubitsyna | |||
*Rapid electrical detection of bacterial growth: Uros Zupancic | |||
*A synthetic model system for biosynthesis of starch-like polysaccharides: Joe White | |||
===Old Projects=== | |||
*[[Cfrench:hemtoppage|Bacteriohemerythrins]]: C. French | *[[Cfrench:hemtoppage|Bacteriohemerythrins]]: C. French | ||
===Protocols=== | *[[Cfrench:BioBrickVectors1|Making a broad host range BioBrick vector]]: Edinburgh iGEM2007 team and C. French | ||
===Outreach=== | |||
*[[Cfrench:ScienceFiction|1. Portrayal of Scientists in Science Fiction]] | |||
===Workshops=== | |||
*[[Cfrench:CaseStudy1|Synthetic Biology Case Study 1: development of an arsenic biosensor]] | |||
*[[Cfrench:CaseStudy2|Synthetic Biology Case Study 2: development of processes for bioconversion of cellulosic materials]] | |||
===Biobrick Protocols=== | |||
*[[Cfrench:bbprimerdesign|1. Primer Design]] | |||
*[[Cfrench:PfuPCR|2. Cloning parts by PCR with Pfu polymerase]] | |||
*[[Cfrench:KodPCR|2b. Cloning parts by PCR with Kod polymerase]] | |||
*[[Cfrench:AGE|3. Agarose Gel Electrophoresis]] | |||
*[[Cfrench:DNAPurification1|4. Purifying a PCR product from solution]] | |||
*[[Cfrench:bbcloning|5. Cloning a PCR product into a biobrick vector]] | |||
*[[Cfrench:compcellprep1|6. Preparing and using competent E. coli cells]] | |||
*[[Cfrench:PCRScreening|7. Screening colonies by PCR]] | |||
*[[Cfrench:minipreps1|8. Plasmid DNA minipreps]] | |||
*[[Cfrench:maxipreps1|8b. Plasmid DNA maxipreps]] | |||
*[[Cfrench:restriction1|9. Analytical restriction digests]] | |||
*[[Cfrench:bbcombining1|10. Combining two biobricks to make a new biobrick]] | |||
*[[Cfrench:addingrbs|11. Adding a ribosome binding site to a biobrick coding sequence]] | |||
*[[Cfrench:BALTIC|12. BALTIC: a new method for combining standard BioBricks]] | |||
*[[Cfrench:MABEL|13. MABEL: a fast easy method for removing unwanted restriction sites]] | |||
*14. BABEL: a new method for generating BioBricks without restriction digests. | |||
===Other Protocols=== | |||
*Growth of E. coli | *Growth of E. coli | ||
*Growth of B. subtilis | *Growth of B. subtilis | ||
*[[Cfrench:BacTrans1|Transforming ''Bacillus subtilis'']] | |||
*[[Cfrench:BacTrans2|Transforming ''Bacillus subtilis'' - 2]] | |||
*[[Cfrench:minipreps1|Plasmid DNA minipreps]] | *[[Cfrench:minipreps1|Plasmid DNA minipreps]] | ||
*Restriction digests | *Restriction digests | ||
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*Cloning of PCR products | *Cloning of PCR products | ||
*Preparation of cell extracts | *Preparation of cell extracts | ||
*Protein Assay | *[[Cfrench:ProteinAssay|Protein Assay]] | ||
*[[Cfrench:sdspage1|SDS-PAGE]] | *[[Cfrench:sdspage1|SDS-PAGE]] | ||
*Native-PAGE | *Native-PAGE | ||
*Enrichment and growth of environmental bacteria | *Enrichment and growth of environmental bacteria | ||
*Identification of bacteria | *[[Cfrench:IdentifyingBacteria|Identification of bacteria]] | ||
*[[Cfrench:gramstain|Gram stain]] | *[[Cfrench:gramstain|Gram stain]] | ||
*[[Cfrench:glycerol|Making glycerol stocks]] | |||
*[[Cfrench:anthrone|Assaying polysaccharides with the anthrone assay]] | |||
*[[Cfrench:Sporulation|Production of ''B. subtilis'' endospores]] | |||
===Lab information=== | |||
[[Cfrench:primerlist|Catalog of primers]] | |||
===Safety Information=== | ===Safety Information=== | ||
[[Cfrench:toxiclist|Hazardous chemicals list]] | [[Cfrench:toxiclist|Hazardous chemicals list]] | ||
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[http://www.biology.ed.ac.uk/people/homepage.php?id=cfrench SBS page] | [http://www.biology.ed.ac.uk/people/homepage.php?id=cfrench SBS page] | ||
[http://www.ed.ac.uk/ University of Edinburgh.] | [http://www.ed.ac.uk/ University of Edinburgh.] | ||
Latest revision as of 14:03, 15 October 2015
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